Then, we transiently transfected melanoma cells with GFP-LC3B plasmids in both A375 and MV3 cells and observed the cells under a fluorescence microscope, and the results presented that leflunomide-treated cells showed more aggregations of GFP-LC3B puncta compared to DMSO-treated groups (Figure 2D). Here, MAP1LC3B is linked to melanoma.