DHODH and melanoma: To further explored whether DHODH downregulation affected apoptosis induced by these drugs, DHODH-silenced human melanoma cells were dealt with 1 nM actinomycin D for 24 h, 0.5 μM doxorubicin for 12 h, or 400 μM temozolomide for 72 h severally (DMSO as the control), then analyzed by flow cytometry.