Supplementary Figure 3A shows that TsCD146 mAb did not bind to HUVEC MP but did bind to UACC MP. In view of these results, we performed a second series of experiments with plasmas from control patients and patients with melanoma (Supplementary Figure 3B). Altogether, results show that annexin V/Ts CD146-positive cancer MP were detected in the plasma of patients with melanoma whereas none were detected in the plasma of control patients. In addition, the number of annexin V/TsCD146-positive MP could be correlated with the initial versus metastatic stages of the tumor (Figure 4). Here, MCAM is linked to cancer.