To examine whether treatment with MLN2238 promotes the accumulation of Mcl-1 in HCC, HepG2, Hep3B, and SNU475 cells were exposed to 125, 250, and 500 nM of MLN2238 treatment for 24 h and with 500 nM for 24 and 48 h. This evidence concerns the gene MCL1 and hepatocellular carcinoma.