We sequenced the cell lines for K-Ras mutations and found that KKU213, HuCCT1, and RBE harbor activated K-Ras mutations, whereas the remaining four CCA cell lines, including KMCH, Huh28, MzCHa1, and OCUG, display wild-type K-Ras alleles (Supplemental Table 1). The gene discussed is KRAS; the disease is cholangiocarcinoma.