Amongst CHD-related genes associated with septal defects (A/V) and ToF, we identified critical cardiac genes (GATA4/6, MYH6/7, MYH7, ACTN2, HAND2, TBX1/5, TDGF1) to be significantly altered in our NIPBL+/− cardiomyocytes. This evidence concerns the gene HAND2 and coronary artery disorder.