BAY 11-7082 reversed pro-metastasis effects of NKILA knockdown in Eca109 and Eca9706 cells (Supplementary Figure S4A), while TNFα blocked the anti-metastasis roles of NKILA reintroduction in KYSE30 and KYSE520 cells (Supplementary Figure S4B), further indicating that NKILA regulated migration of ESCC cells via NF-κB pathway. Here, TNF is linked to esophageal squamous cell carcinoma.