IRF8 and acute myeloid leukemia: Similar analyses using a separate, smaller dataset from TCGA also gave similar results (Figure S5C), in particular with regard to expression levels of GFI1 and IRF8. Together these data demonstrate that although IRX3 and FOXC1 misexpression contribute to the differentiation block observed in human AML, they repress myelomonocytic lineage transcription factor genes in a distinctive manner: FOXC1 represses monocytic lineage genes more profoundly than does IRX3, whereas the opposite is the case for GFI1.