To evaluate the consequences of misexpressed IRX3 and FOXC1 on expression levels of transcription factors required for normal myelomonocytic lineage differentiation, and to determine why the phenotypic consequences of IRX3 and FOXC1 misexpression differed one from another, we performed qPCR using murine leukemia samples and analyzed expression levels in published human AML datasets. The gene discussed is IRX3; the disease is leukemia.