GNAI1 and Anxiety: Silencing CRH neurons using Gi did not significantly alter anxiety-like behavior in the open field (17.4 ± 2.0% time in center) or light-dark box (31.6 ± 2.8% time in light) compared to GFP-injected controls (13.2 ± 2.2% time in center; 22.0 ± 6.3% time in light) (Fig 5b).