Further studies in mice of the same group demonstrated that secreted Klotho promoted endothelial increase of NO in aorta and arterioles [40], and that adenovirus-mediated Klotho gene delivery to a typical rat model of multiple atherogenic risk (OLETF rat) improved endothelial dysfunction, increased NO production, reduced increased blood pressure, and prevented medial hypertrophy, meaning that Klotho was a clear positive regulator of vascular function [41]. Here, KL is linked to endothelial dysfunction.