For instance, in anorexia nervosa patients, in which the existence of an increased pulsatile secretion of GH is well known, while plasma levels of IGF-I are low or very low, due to malnutrition, plasma levels of Klotho are lower than expected for the age of the patients [50], but they increased significantly after the patients increased their body weight and, concomitantly IGF-I increased too. Here, IGF1 is linked to nutritional deficiency disease.