In agreement with the highly malignant and invasive Rn tumor, Rn derived cell line also displayed a more aggressive phenotype by forming respectively 2-, 2- and 4-fold more colonies in soft agar; and 3-, 3- and 7- fold more adherent colonies than Dc, Bsk and Rk. Accordingly, Rn cell line displayed the greatest proportion of cells with lower basal ROS levels, lower size and higher complexity (Table 1). The gene discussed is EPHA5; the disease is neoplasm.