Further research found that in the HCC rat model induced by diethylinitrosamine (DEN), the aqueous extract of Astragali radix combined with Salvia miltiorrhiza reduced the incidence and multiplicity of HCC development in a dose-dependent manner by inhibiting fibrosis and the transcription of plasminogen activator inhibitor-1 [45,46]. This evidence concerns the gene SERPINE1 and hepatocellular carcinoma.