Moreover, in orthotopic HCC mouse model, AST-IV significantly reduced the tumor weight, decreased the count of tumor microvessels and the expression of angiogenic factors including MMP2, FGF2, VEGF, and HGF, which might be due to up-regulating a tumor suppressor gene miR-122, while down-regulating an oncogene miR-221 [114]. This evidence concerns the gene MMP2 and neoplasm.