We then investigated the methylation level of each CpG site or cluster in the promoters of the other 13 DEGs verified by qPCR (ANGPTL4, CD9, NR4A1, EDN2, PTX3, LIF, SLC12A8, PEX3, CYP17A1, SERPINE1, DIRAS3, BNIP3, SPP1) in 16 GC DNA samples (8 PCOS women and 8 controls, the first cohort). The gene discussed is NR4A1; the disease is polycystic ovary syndrome.