TP53 and colorectal adenocarcinoma: To this end, the colorectal adenocarcinoma-derived cell line, SW480, endogenously expressing mutant p53R273H,P309S, was stably introduced with either shRNA against p53 (shp53) to knock-down mutant p53R273H,P309S expression, or as a controls with shRNA against nonspecific sequence (shCon) (Fig. 1a; Supplementary Figure 1, Supplementary Figure 2A) as well as shRNA control against lacZ gene (sh-lacZ) (Supplementary Figure 2A).