In the present study, we demonstrated that adenovirus-mediated TIPE2 overexpression suppressed the expression of β-catenin in EC9706 cells and xenograft tumors of esophageal carcinoma, indicating that TIPE2 acted as a tumor suppressor by inactivating the Wnt/β-catenin pathway in esophageal carcinoma. This evidence concerns the gene TIPE2 and carcinoma of esophagus.