For further investigating the clinical correlation of NDC80 in CRC patients, immunohistochemical staining combined with semiquantitative evaluation was employed and the representative results were demonstrated in Figure 1D. The ROC curve analysis suggests the cut‐off value of staining scores is 3.5 (Fig. 1E), and therefore the entire patient cohort was classified into high expression group (n = 108) and low expression group (n = 116) based on it. This evidence concerns the gene NDC80 and colorectal carcinoma.