All the anti-neoplastic drugs employed, increased CAV1 expression in the colon cancer cell line HT29(US), but the effect was stronger with the dihydrofolate reductase inhibitor Methotrexate, and the topoisomerase II inhibitor Etoposide (Figure 2A, Supplementary Figure 1A and 1B). The gene discussed is CAV1; the disease is malignant colon neoplasm.