Inhibition of MEK and pre-treatment with Trolox, both individually precluded CAV1 up-regulation induced by either Methotrexate or Etoposide in colon cancer cell lines HT29(US) (Figure 5A), DLD-1 (Figure 5B) and HT29(ATCC) (Supplementary Figure 3A), as well as in MCF7 breast cancer cells (Supplementary Figure 3B). This evidence concerns the gene CAV1 and malignant colon neoplasm.