All the anti-neoplastic drugs employed, increased CAV1 expression in the colon cancer cell line HT29(US), but the effect was stronger with the dihydrofolate reductase inhibitor Methotrexate, and the topoisomerase II inhibitor Etoposide (Figure 2A, Supplementary Figure 1A and 1B). Here, DHFR is linked to malignant colon neoplasm.