TWIST1 and hepatocellular carcinoma: In addition, the expression of IGF-1R was significantly positively associated with the expression of N-cadherin, Vimentin, Snail1, Snail2, and Twist1 (all p<0.05) (Figure 1b-1f), which indicates that IGF-1/IGF-1R axis seems to be involved in EMT of human HCC.