Therefore, to validate the candidate targets in the network in bladder cancer tissues, we sorted tissue cells based on their level of expression of the CD44 marker and performed qRT-PCR to examine the level of mRNA expression of three genes (BAX, SLC39A6, and ITGB1), as well as the three key TFs (ETS1, MYC, and EGR1) in the network. Here, EGR1 is linked to urinary bladder cancer.