EGR1 and urinary bladder carcinoma: Therefore, to validate the candidate targets in the network in bladder cancer tissues, we sorted tissue cells based on their level of expression of the CD44 marker and performed qRT-PCR to examine the level of mRNA expression of three genes (BAX, SLC39A6, and ITGB1), as well as the three key TFs (ETS1, MYC, and EGR1) in the network.