Our data suggest that the increase in CD4+ T cell response is an indirect effect of Rspo2, for example, due to loss of epithelial function and in the mucosal barrier, rather than a direct consequence of Rspo2 on immune cells since we did not observe increases in active β-catenin in immune cells of the colonic lamina propria during infection (data not shown). The gene discussed is CD4; the disease is infection.