To demonstrate the significance of EVI1 on SLUG expression, we overexpressed EVI1 in EVI1 negative HCT15 (stage III, non-metastatic) colon cancer cell line and did matrigel based invasion assay and observed that EVI1 reduces the invading capacity of HCT15 cells in comparison to empty vector transfected cells (Fig. 4c). The gene discussed is MECOM; the disease is malignant colon neoplasm.