Traditional flow cytometric analysis in FlowJo revealed that LAG-3, 2B4, and PD-1 were all significantly upregulated on CD4+ T cell populations in cancer septic mice relative to previously healthy control mice (Fig 4A and 4B), resulting in significant increases in the absolute numbers of CD4+ T cells expressing these phenotypic markers of exhaustion (Fig 4C). This evidence concerns the gene CD4 and cancer.