MIF and stroke disorder: Further evaluation of cerebrovascular integrity also revealed that MIF-treated group (10 μg/kg, 3 hours post pMCAo) exhibited a marked increase of dye content in the extravascular compartment of the ischemic hemisphere as compared with control stroke animals, indicating an increase of BBB permeability among MIF-treated animals (Fig. 6C, 1.4 ± 0.2 vs 5.9 ± 0.9 μg/g tissue).