These findings were inconsistent with an earlier report that OCT4 protein was lost from hPSCs maintained on cRGDfK-presenting culture surfaces10 Furthermore, each hPSC culture passed teratoma assays for the in vivo formation of tissues representing the three embryonic germ layers (Fig. 6B, Supporting Information Figure S9) and the PluriTestTM pluripotency assay; all samples produced Novelty Scores and Pluripotency Scores typical of hPSC populations (Fig. 7C). This evidence concerns the gene POU5F1 and teratoma.