ACTA1 and hepatocellular carcinoma: To further confirm the localization of miR-1247-3p expression in HCC tissue and lung metastatic tissue sample, in situ hybridization of miR-1247-3p a in combination with immunohistochemistry (IHC) staining of HCC markers (AFP) and fibroblast markers (α-SMA) were performed on serial sections of human HCC tissues and lung metastatic tissues.