Similarly, mRNA sequencing in EZH2 knockdown cells showed a good evidence for EZH2’s regulation on cell stemness at the molecular level (Fig. 3c), and further validation showed that protein expression levels of the pluripotent genes, Oct4 and Sox2, were both significantly upregulated in Ezh2-overexpressing cells, whereas significantly reduced in Ezh2-knockdown GC cells compared with the control group (P < 0.01, Fig. 3d). The gene discussed is POU5F1; the disease is gastric cancer.