In total, 8 genes, including MUC16, UNC79, USH2A, DNAH17, PTPN13, TENM4, PCLO, and PDE1C, were found to be mutated in at least 2 HCC samples (Table 2); 17 candidate somatic mutations located on these genes were further confirmed via PCR-based Sanger sequencing (Figure 2, Table S6). This evidence concerns the gene DNAH17 and hepatocellular carcinoma.