To assess the presence of a CSC subpopulation in human melanoma cell lines, A375 and BLM cells were plated as single cell-suspension and cultured in serum-free Euromed-N medium supplemented with growth factors (10 ng/ml EGF and 10 ng/ml FGF-2) and 1% N2; their ability to form melanospheres was followed and images were taken after 5 and 15 days of culture. The gene discussed is EGF; the disease is melanoma.