Using data in the literature that identified surface markers highly expressed in glioblastoma cell lines, EVs and primary tumors27, antibodies directed against EGFR, EGFRvIII, ephA2, podoplanin, PDGFR, and MCAM were screened for specificity and sensitivity with our microfluidic device. The gene discussed is PDGFRB; the disease is glioblastoma.