Significant over expression of SHH (R.E = 4.4 ± 3.32), DHH (R.E = 4.7 ± 3.46), PTCH1 (R.E = 3.4 ± 3.08) and GLI1 (R.E = 3.8 ± 2.49) were detected in the breast tumour biopsies as compared with controls. This evidence concerns the gene SHH and breast neoplasm.