At the protein level, a JUP induction in the FSHD sample or following DUX4 or DUX4c expression was less clear (stars in Additional file 5: Figure S5B), even though the gene encoding JUP has been described as a direct DUX4 target [14], and its mRNA level rapidly increases upon DUX4 induction in C2C12 cells [13]. This evidence concerns the gene DUX4 and facioscapulohumeral muscular dystrophy.