Accordingly, under our culture conditions, which were identical to those of Barro et al. [22] and differed from those of other studies in FSHD muscle cells, we observed higher levels of DUX4 in aFSHD than in dFSHD myotubes [16] and high DUX4c levels in dFSHD myotubes ([28] and this study). Here, DUX4 is linked to facioscapulohumeral muscular dystrophy.