Betaine supplemented HDM-treated C57BL/6Mthfr-/- compared to HDM-treated C57BL/6Mthfr-/- mice that were not supplemented with betaine exhibited a significant increase in cellularity and eosinophilia in the WLL without an effect on AHR (Fig 7B–7E). This evidence concerns the gene AHR and Increased total eosinophil count.