In addition, early treatment with Leu induced a reduction in the expression of Tsc1 and in the proline-rich substrate of AKT1 (Akt1s1 also called PRAS40), the latter accentuated by HF-diet; a decreased expression of Akt2 was also found in L-animals under the HC-diet. The gene discussed is AKT1S1; the disease is hydrops fetalis.