TSHR and hypothyroidism: Transgenic BALB/c mice expressing low intrathyroidal levels of the human TSHR A-subunit, depleted of regulatory T cells (CD25 positive) before immunization with human TSHR-A-subunit adenovirus, developed TSHR antibodies and (unexpectedly) TgAb- and TPOAb-associated with massive thyroiditis and hypothyroidism (90).