In addition, there was no significant difference in the frequency of Foxp3+ liver-infiltrating CD4+ T cells between the cGVHD and THSC-depleted groups, suggesting that the lethal exacerbation of GVHD in the THSC-depleted group could not be attributed to the loss of Foxp3+ regulatory T cells (Figure 5H). This evidence concerns the gene CD4 and graft versus host disease.