BIRC5 and breast cancer: Here, results of the Western blot analysis showed that pharmacological inhibition of mTOR by rapamycin decreased the expression of survivin and p62/SQSTM1 (autophagic flux indicator) in MCF7, MCF7-TamC3, and ZR-75-1 cells, confirming that mTOR positively modulates survivin expression and negatively regulates autophagy in the tested ER+ breast cancer cells (Supplementary Figure S2).