The results consistently showed that, compared to infection with WT virus, type I IFN (IFA 1/13, IFNB1), type III IFN (IL28B), and ISGs (IRF7, IFIH1 and IFITM1) are strongly upregulated in both MARC-145 cells and swine macrophages infected with the vKO1 and vKO2 mutants, which further implicates nsp2TF/nsp2N in innate immune suppression. This evidence concerns the gene IFIH1 and infection.