As expected, a 7-week high fat diet (HFD) feeding induced early-stage liver pathologies of NAFLD in rat, including fatty liver and liver injury, which is manifested by increased plasma ALT, AST, TC, TG, LDL-C levels (Fig 3F) and decreased plasma HDL-C levels (Fig 3E) respectively. Here, GPT is linked to metabolic dysfunction-associated steatotic liver disease.