As c-Myc is known to be an important regulator of cancer metabolism, especially glutamine metabolism22, in examining the relationship between c-Myc and glutamine metabolism in our experiments, we performed Western-blot analysis for c-Myc, GLS1, and ASCT2 in eight CRC cell lines, and c-Myc knockdown experiments in SW620 cells. This evidence concerns the gene SLC1A5 and colorectal carcinoma.