NLRP3 and age-related macular degeneration: Although none of the previously used NLRP3 antibodies in AMD research met all validation criteria, a recently developed monoclonal anti-NLRP3 antibody (CST, D4D8T, 15101) (Fig. 1i,j) which has not been used in previously published AMD-related research, met all three criteria for specificity, sensitivity and signal induction.