RB1 and cancer: Importantly, a study employing next-generation sequencing of 1,000 cancer-associated genes from primary lesions, pulmonary metastases, and normal central nervous system tissues, identified mutations in the EGFR, RB transcriptional corepressor 1 (RB1) and SET domain containing 2 (SETD2) genes in the metastatic site, but not in the primary tumor site [9].