While low COX-2 expressing MCF7 and T47D human breast cancer cells showed significant increases in miR655 expression with PGE2 and PGE1OH treatments (Fig. 6D), above treatments could not increase miR655 expression in MDA-MB-231 cells, which already had high expression of COX-2 and miR655. The gene discussed is PTGS2; the disease is breast cancer.