Moreover, we observed an increase in Blue area (Cells) at all timepoints compared to WT littermates, but did not find an increase in Pink area (ECM, Blood, Cytoplasm, ect) at 4, 5.5 or 12 months of age, indicating that the ILD in TNF-Tg mice is primarily inflammatory and not fibrotic (Fig 4H and 4I). Here, TNF is linked to interstitial lung disease.