IFNG and tuberculosis: IFN-γ can promote the formation of the Class II major histocompatibility complex (MHC-II), and T-SPOT.TB can be used to detect IFN-γ and other cytokines at the same time, such as interleukin-2 (IL-2) (13), IL-4, IL-5, IL-6, IL-10, etc., and improve the sensitivity and specificity of detection.