To determine if BCL2 downregulation is necessary for MYBMIM-induced apoptosis of AML cells, we expressed BCL2 using MSCV-IRES-GFP (MIG) retrovirus in MV-411 cells, and confirmed its ectopic overexpression using qRT-PCR (Supplementary Fig. 10). Here, BCL2 is linked to acute myeloid leukemia.