To further explore whether NF-κB was involved in H. pylori-mediated upregulation of miR-223-3p, we used a specific NF-κB signal inhibitor BAY 11-7082 to treat the cells and found that NF-κB inhibitor treatment reversed H. pylori (CagA+) infection or CagA expression vector transfection-mediated upregulation of miR-223-3p. The gene discussed is NFKB1; the disease is infection.