Immunohistochemistry, Western blot analysis, and RT-PCR have been used to detect HSP70, PI3K, and AKT protein and mRNA expression in liver cancer tissues and analyzed the association with gender, age, histologic differentiation, lymph node metastasis, and tumor, lymph node, metastasis (TNM) staging, and other clinical pathology parameters. This evidence concerns the gene AKT1 and neoplasm.