PLA detection of endogenous BAG3/HSPB8 complexes generated a more abundant signal in DMD cell lines than in control cells; indeed, the average number of dots (representing a single protein complex) per cell was increased by 2.7 (D1) to 4.5-fold (D4) in DMD myoblasts compared to controls (Figure 5C, graph). This evidence concerns the gene BAG3 and Duchenne muscular dystrophy.