The autophagy process in solid tumors was validated by immunochemistry of LC3B and Beclin‐1 as well as Western blotting of Beclin‐1, in which the expression of both indicators was upregulated by TAN administration (Fig. 6B–D), confirming the tumor‐suppressing effect of TAN in vivo. The gene discussed is MAP1LC3B; the disease is neoplasm.