ASCL2 and malignant colon neoplasm: To confirm whether the YAP1-enhanced self-renewability of colon cancer progenitor cells was related to a KLF5-dependent Ascl2 increase, the relative ‘stemness’-associated genes expression (mRNA) levels and protein levels in lv-YAP1/HT-29 and lv-YAP1/Caco-2 cells were determined, and they were found to be significantly increased compared to their respective control cells (Figure 5).