To this end, a pair of small hairpin RNAs (shRNAs) specifically against PKM2 (shPKM2#1) was transfected into MCF7 (Figure 2A, Supplementary Figure 1A and 1B) and human lung cancer cell A549 (Supplementary Figure 1C), together with a non-specific shRNA as a negative control (NC). Here, PKM is linked to lung cancer.